Tags :Spectronaut

Spectronaut 20 is a powerful software package developed by Biognosys for analyzing data-independent acquisition (DIA) proteomics experiments. It is designed to deliver high-performance analysis with enhanced speed, depth, and scalability, particularly for complex proteomic datasets. Below is an overview based on available information: Key Features of Spectronaut 20: Kuiper Search Engine: Introduces a new search engine that significantly boosts speed and depth, achieving ~70% faster runtimes and ~75% more HLA class I peptide identifications compared to Spectronaut 19. It excels in complex search spaces like unspecific digests and PTM (post-translational modification) discovery. directDIA Workflow: Streamlines immunopeptidomics by outperforming library-based approaches, identifying ~40% more HLA type I peptides on average. This eliminates the need for extensive spectral library creation, saving time...

Spectronaut is a Data Acquisition for Independent Analysis (DIA) proteomics experiment designed to. 1. Building hybrid libraries By combining large-scale spectral libraries from other sources (project or resource libraries) with DIA data from the samples themselves (directDIA), it is possible to obtain large quantitative protein counts without having to build the libraries yourself. 2. Labeling Workflow With Biognosys’ new PQ500 Isotope Labeled Peptide Kit, target proteins can be quantified absolutely and across 5 orders of magnitude by off-label quantification. 3. Host Cell Proteins Low-abundance host cell proteins can be characterized and quantified with high precision by correcting for residual proteins.